Explain presence of Carcinoembryonic antigen (CEA) in liver cells of a patient suffering from a liver tumor
Case study in which you need to decide on the steps and techniques to use to identify the presence of Carcinoembryonic antigen (CEA) in liver cells of a patient suffering from a liver tumor.
You must include the method of collecting the tissue, fixation, processing and staining techniques that are appropriate.
You have been provided with some patient data relating to the case history.
You will need to research and provide a rational for the selection of the various techniques for the pathology investigations conducted
You should answer the questions at the end of the case details
Word count must be stated
Double line spacing should be used
References (textbook and journals) should be embedded and in Harvard format
CASE:
A 54 year old man has come to the hospital suffering from liver problems. The patient had a colon carcinoma removed 3 years ago. The liver is slightly swollen and an MRI scan reveals a dense mass in the middle of the right lobe. The doctors suspect that this is metastasis and recurrence of the colon cancer. They consult you to check what they should do to help you reach an accurate histopathology diagnosis. Questions to answer in your assignment
1) What method is most suitable to remove adequate tissue for a histopathology examination and what is the most reliable way to ensure you get the correct tissue needed?
2) What methods of fixation and processing are most suitable for the analyses you are thinking of performing?
3) What possible techniques could you perform on the sample of tissue obtained, to help in the microscope examination, bearing in mind the methods you use need to confirm the presence or absence of CEA.
4) What other staining methods can or should you use as well? NOTE: You must explain the reasons for your choices and the underlying principles of the methods and techniques you select.
Answer preview for the explain presence of Carcinoembryonic antigen (CEA) in liver cells of a patient suffering from a liver tumor
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